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    28 July 2021, Volume 28 Issue 4 Previous Issue    Next Issue

    Letter
    Review
    Research Papers
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    Letter
    SDF5 Encoding P450 Protein Is Required for Internode Elongation in Rice
    Yachun Yang, Juan Li, Hao Li, Zuntao Xu, Ruiying Qin, Wenge Wu, Pengcheng Wei, Yong Ding, Jianbo Yang
    2021, 28(4): 313-316.  DOI: 10.1016/j.rsci.2021.05.001
    Abstract ( )   HTML ( )   PDF (694KB) ( )  
    RGB1 Regulates Rice Panicle Architecture and Grain Filling Through Monitoring Cytokinin Level in Inflorescence Meristem and Grain Abscisic Acid Level During Filling Stage
    Dongping Zhang, Minyan Zhang, Yuzhu Wang, Jiansheng Liang
    2021, 28(4): 317-321.  DOI: 10.1016/j.rsci.2021.05.002
    Abstract ( )   HTML ( )   PDF (678KB) ( )  
    Development of Chromosome Segment Substitution Lines and Genetic Dissection of Grain Size Related Locus in Rice
    Riaz Aamir, Huimin Wang, Zhenhua Zhang, Anley Zegeye Workie, Yanhui Li, Hong Wang, Pao Xue, Zequn Peng, Xihong Shen, Shihua Cheng, Yingxin Zhang
    2021, 28(4): 322-324.  DOI: 10.1016/j.rsci.2021.05.003
    Abstract ( )   HTML ( )   PDF (474KB) ( )  
    Review
    Application of Rice Husk Biochar for Achieving Sustainable Agriculture and Environment
    Asadi Hossein, Ghorbani Mohammad, Rezaei-Rashti Mehran, Abrishamkesh Sepideh, Amirahmadi Elnaz, Chengrong Chen, Gorji Manouchehr
    2021, 28(4): 325-343.  DOI: 10.1016/j.rsci.2021.05.004
    Abstract ( )   HTML ( )   PDF (931KB) ( )  

    This paper critically reviewed the current knowledge and challenges of rice husk biochar (RHB) production and its effects on soil properties, plant growth, immobilization of heavy metals, reduction of nutrient leaching and mitigation of greenhouse gas emissions. The characteristics of RHBs produced at various pyrolysis temperatures were discussed and compared to biochars derived from other agro- industrial wastes. RHBs produced at higher pyrolysis temperatures show lower hydrogen/carbon ratio, which suggests the presence of higher aromatic carbon compounds. The increase of pyrolysis temperature also results in production of RHBs with higher ash content, lower yield and higher surface area. RHB usually has higher silicon and ash contents and lower carbon content compared to biochars derived from other feedstocks at the same pyrolysis conditions. Although it depends on soil type, RHB application can improve soil organic carbon content, cation exchange capacity, available K concentration, bulk density and microbial activity. The effect of RHB on soil aggregation mainly depends on soil texture. The growth of different crops is also enhanced by application of RHB. RHB addition to soil can immobilize heavy metals and herbicides and reduce their bioavailability. RHB application shows a significant capacity in reduction of nitrate leaching, although its magnitude depends on the biochar application rate and soil biogeochemical characteristics. Use of RHB, especially in paddy fields, shows a promising mitigation effect on greenhouse gas (CH4, CO2 and N2O) emissions. Although RHB characteristics are also related to other factors such as pyrolysis heating rate and residence time, its performance for specific applications (e.g. carbon sequestration, pH amendment) can be manipulated by adjusting the pyrolysis temperature. More research is needed on long-term field applications of RHB to fully understand the advantages and disadvantages of RHB as a soil amendment.

    Research Papers
    MULTI-FLORET SPIKELET 4 (MFS4) Regulates Spikelet Development and Grain Size in Rice
    Yan Wang, Xiaoqin Zeng, Lu Lu, Qinglan Cheng, Fayu Yang, Mingjiang Huang, Mao Xiong, Yunfeng Li
    2021, 28(4): 344-357.  DOI: 10.1016/j.rsci.2021.05.005
    Abstract ( )   HTML ( )   PDF (1726KB) ( )  

    In rice, the spikelet is the basic unit of inflorescence, and its development is important for determining the grain yield and quality. We reported a rice spikelet mutant multi-floret spikelet 4 (mfs4) which resulted in the production of extra floral organs or a whole extra floret, and elongated sterile lemmas. The results suggested that the mutation of the MFS4 gene interfered with spikelet meristem determinacy and floral organ identity. In addition, the plant height and the grain length and width in the mfs4 mutant were all less than those in the wild type. Using the bulked segregant analysis method, the MFS4 gene was localized in a 557-kb region on the long arm of chromosome 1. Sequence analysis showed that there was a C-base deletion at the open reading frame of LOC_Os01g67430. Further tests indicated that a wild type copy of LOC_Os01g67430 was able to reverse the mfs4 defects, which indicated that LOC_Os01g67430 was the MFS4 gene. The MFS4 gene encodes a lipase located in the mitochondria and is expressed strongly in the young inflorescence. qRT-PCR results showed that the expression of some genes that were known to regulate spikelet meristem determinacy and grain size were decreased in the mfs4 mutant, which indicated that the MFS4 gene regulates spikelet meristem determinacy and grain size by modulating the expression of these genes.

    OsbZIP09, a Unique OsbZIP Transcription Factor of Rice, Promotes Rather Than Suppresses Seed Germination by Attenuating Abscisic Acid Pathway
    Chuxin Wang, Chengchao Zhu, Yu Zhou, Min Xiong, Jindong Wang, Huang Bai, Chenya Lu, Changquan Zhang, Qiaoquan Liu, Qianfeng Li
    2021, 28(4): 358-367.  DOI: 10.1016/j.rsci.2021.05.006
    Abstract ( )   HTML ( )   PDF (1077KB) ( )  

    We successfully identified a novel and unique OsbZIP transcription factor, OsbZIP09, whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type, but shared similar germination rate as the wild type under normal germination conditions. The expression of OsbZIP09 was induced by abscisic acid (ABA) and declined as the germination process. As a nucleus-localized transcription factor, the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique. Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1, thus reducing ABA accumulation. In addition, OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression, thus further alleviating the suppressive effect of ABA on seed germination. These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies.

    A New Approach to Select Doubled Haploid Rice Lines under Salinity Stress Using Indirect Selection Index
    Fuad Anshori Muhammad, Sapta Purwoko Bambang, Saraswati Dewi Iswari, Wahyuning Ardie Sintho, Bayuardi Suwarno Willy
    2021, 28(4): 368-378.  DOI: 10.1016/j.rsci.2021.05.007
    Abstract ( )   HTML ( )   PDF (948KB) ( )  

    This study determined the indirect selection index of doubled haploid (DH) rice using a multivariate analysis approach to select lines adaptive to salinity stress, comprising three experiments. The first experiment involved the selection of good agronomic characters in a field experiment conducted at an experimental station in Bogor, Indonesia. The second experiment involved salinity tolerance screening through hydroponic cultures using 0 and 120 mmol/L NaCl, conducted at a greenhouse in Bogor. The third experiment involved the validation of the indirect adaptability selection index (IASI) through a field experiment in Sukra (saline area). Field experiments followed a randomized complete block design (RCBD), whereas an RCBD nested factorial design was used for the greenhouse experiment. The first and second experiments used 56 DH lines and four check varieties with three replications. In the second experiment, Pokkali and IR29 varieties were also added as tolerant and sensitive checks of salinity, respectively. The third experiment used 28 selected DH lines, Inpari 29 and one sensitive DH line. The good agronomic index (GAI) was 0.465 yield + 0.433 number of productive tillers + 0.31 number of filled grains. This generated 24 DH rice lines with good agronomic traits. The salinity tolerance index (SaTI) was developed through the average of standardized salinity tolerance score and salinity selection index based on discriminant analysis. This generated 34 DH rice lines with good salinity stress tolerance. The IASI (IASI = GAI - SaTI) selected 28 DH rice lines adaptive to salinity stress and it was considered effective by Sukra validation.

    Genome-Wide Association Study of Nitrogen Use Efficiency and Agronomic Traits in Upland Rice
    Rakotoson Tatiana, Dusserre Julie, Letourmy Philippe, Frouin Julien, Ramonta Ratsimiala Isabelle, Victorine Rakotoarisoa Noronirina, Cao Tuong-Vi, Vom Brocke Kirsten, Ramanantsoanirina Alain, Ahmadi Nourollah, Raboin Louis-Marie
    2021, 28(4): 379-390.  DOI: 10.1016/j.rsci.2021.05.008
    Abstract ( )   HTML ( )   PDF (817KB) ( )  

    Genome-wide association study (GWAS) was performed for 16 agronomic traits including nitrogen use efficiency (NUE) and yield-related components using a panel of 190 mainly japonica rice varieties and a set of 38 390 single nucleotide polymorphism (SNP) markers. This panel was evaluated under rainfed upland conditions in Madagascar in two consecutive cropping seasons with two contrasted nitrogen input levels. Using another set of five grain traits, we identified previously known genes (GW5, GS3, Awn1 and Glabrous1), thus validating the pertinence and accuracy of our datasets for GWAS. A total of 369 significant associations were detected between SNPs and agronomic traits, gathered into 46 distinct haplotype groups and 28 isolated markers. Few association signals were identified for the complex quantitative trait NUE, however, larger number of quantitative trait loci (QTLs) were detected for its component traits, with 10 and 2 association signals for nitrogen utilization efficiency and nitrogen uptake efficiency, respectively. Several detected association signals co-localized with genes involved in nitrogen transport or nitrogen remobilization within 100 kb. The present study thus confirmed the potential of GWAS to identify candidate genes and new loci associated with agronomic traits. However, because of the quantitative and complex nature of NUE-related traits, GWAS might have not captured a large number of QTLs with limited effects.

    iTRAQ-Based Proteomics Investigation of Critical Response Proteins in Embryo and Coleoptile During Rice Anaerobic Germination
    Guangchen Zhang, Zimeng Liu, Youhong Liu, Noriyuki Kuya, Yuchen Hua, Hongru Shi, Weilin Zhao, Yuqing Han, Toshio Yamamoto, Wenfu Chen, Jian Sun
    2021, 28(4): 391-401.  DOI: 10.1016/j.rsci.2021.05.009
    Abstract ( )   HTML ( )   PDF (831KB) ( )  

    Direct-seeding of rice has become popular in recent years due to its low cost and convenience, however, hypoxic condition limits seedling establishment. In this study, weedy rice WR04-6 with high germination ability under anaerobic conditions was used as a gene donor, and we successfully improved the seedling establishment rate of rice cultivar Qishanzhan (QSZ) based on selection of a new rice line R42 from the recombinant inbred line population. R42 inherited high anaerobic germination (AG) ability, and was used for isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic studies with QSZ to further explore the molecular mechanism of AG. A total of 719 differentially abundant proteins (DAPs) were shared by R42 and QSZ responded to AG, and thus defined as common response DAPs. A total of 300 DAPs that responded to AG were only identified from R42, which were defined as tolerance-specific DAPs. The common response and tolerance-specific DAPs had similar biochemical reaction processes and metabolic pathways in response to anoxic stress, however, they involved different proteins. The tolerance-specific DAPs were involved in amino acid metabolism, starch and sucrose metabolism, tricarboxylic acid cycle pathway, ethylene synthesis pathway, cell wall-associated proteins and activity of active oxygen scavenging enzyme. The in silico protein-protein interactions for the top 60 DAPs indicated that tolerance-specific DAPs had relatively independent protein interaction networks in response to an anoxic environment compared with common response DAPs. The results of physiological indicators showed that α-amylase and superoxide dismutase activities of R42 were significantly increased under anoxic conditions compared with aerobic conditions. Multiple lines of evidence from western blot, physiological analysis and quantitative real-time PCR jointly supported the reliability of proteomics data. In summary, our findings deepened the understanding of the molecular mechanism for the rice response to AG.

    Effect of Milling and Parboiling Processes on Arsenic Species Distribution in Rice Grains
    Chiara Fontanella Maria, Martin Maria, Tenni Daniele, Maria Beone Gian, Romani Marco
    2021, 28(4): 402-408.  DOI: 10.1016/j.rsci.2021.05.010
    Abstract ( )   HTML ( )   PDF (883KB) ( )  

    This study identified the role of milling and parboiling on arsenic (As) content and its species in large numbers of rice samples. Total As contents were 108 ± 33 μg/kg in polished rice grains (PR), 159 ± 46 μg/kg in unpolished rice grains (UR), 145 ± 42 μg/kg in parboiled polished rice grains (PPR) and 145 ± 44 μg/kg in parboiled unpolished rice grains (PUR). The percentages of inorganic As (iAs) were 66% ± 8% in PR and from 72% to 77% in other grain categories. The polishing process reduced the As content in the rice grains, removing outer part of the UR with high amount of As, whereas the parboiling technique transferred the semimetal content within the grain. Total As and iAs contents were not significantly different in UR, PPR and PUR, homogenizing its distribution inside the grains. The results allowed to understand how different operations affect As fate and its chemical forms in grains.

    Water Management for Improvement of Rice Yield, Appearance Quality and Palatability with High Temperature During Ripening Period
    Matsue Yuji, Takasaki Katsuya, Abe Jun
    2021, 28(4): 409-416.  DOI: 10.1016/j.rsci.2021.05.011
    Abstract ( )   HTML ( )   PDF (823KB) ( )  

    To clarify the optimal water management in large-scale fields under high temperatures at the ripening period, effective water managements during this period for improvement of yield, appearance quality and palatability were investigated. Compared with intermittent irrigation and flooded irrigation, the soil temperature with saturated irrigation remained low throughout the day, and the decrease rate of the bleeding rate of hills was the lowest. These results suggested that the saturated irrigation maintained root activity. For the three irrigation types, the number of spikelets per m2 and 1000-grain weight were similar, however, saturated irrigation resulted in significantly higher rice yield due to improvement in the percentage of ripened grains. The saturated irrigation produced a high percentage of perfect rice grains and thicker brown rice grain, furthermore, the palatability of cooked rice was excellent because protein content and hardness/adhesion ratio were both low. Thus, under high-temperature ripening conditions, soil temperature was lowered and root activity was maintained when applying saturated irrigation after heading time. The results indicated that saturated irrigation is an effective countermeasure against high-temperature ripening damage.