Mapping of qTGW1.1, a Quantitative Trait Locus for 1000-Grain Weight in Rice (Oryza sativa L.)

doi:10.1016/S1672-6308(14)60279-1

Abstract

Abstract:

1000-grain weight (TGW) is one of the three component traits of the grain yield in rice (Oryza sativa L). This study was conducted to validate and fine-map qTGW1.1, a minor QTL for TGW which was previously located in a 3.7-Mb region on the long arm of rice chromosome 1. Five sets of near isogenic lines (NILs) were developed from two BC₂F₄ populations of the indica rice cross Zhenshan 97³/Milyang 46. The NIL sets consisted of two homozygous genotypic groups differing in the regions RM11448-RM11522, RM11448-RM11549, RM1232-RM11615, RM11543-RM11554 and RM11569-RM11621, respectively. Four traits, including TGW, grain length, grain width and heading date, were measured. Phenotypic difference between the two genotypic groups in each NIL population was analyzed using SAS procedure GLM. Significant QTL effects were detected on TGW with the Zhenshan 97 allele increasing grain weight by 0.12 g to 0.14 g and explaining 8.30% to 15.19% of the phenotypic variance. Significant effects were also observed for grain length and width, whereas no significant effect was found for heading date. Based on comparison among the five NILs on the segregating regions and the results of QTL analysis, qTGW1.1 was delimited to a 376.9-kb region flanked by DNA markers Wn28382 and RM11554. Our results indicate that the effects of minor QTLs could be steadily detected in a highly isogenic background and suggest that such QTLs could be utilized in the breeding of high-yielding rice varieties.

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Key words: 1000-grain weight, minor effect, quantitative trait locus, rice (Oryza sativa L.)

Hong-wei Zhang, Yu-yu Chen, Jun-yu Chen, Yu-jun Zhu, De-run Huang, Ye-yang Fan, Jie-yun Zhuang. Mapping of qTGW1.1, a Quantitative Trait Locus for 1000-Grain Weight in Rice (Oryza sativa L.)[J]. Rice Science, 2015, 22(1): 9-15.

Figures/Tables 5

References 25

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Generation	NIL name	Segregating region	Sample for two homozygous genotypes	Growing season
BC₂F_8:9	C901	RM11448-RM11522	24 lines for ZS97, 26 lines for MY46	May-September, 2012
BC₂F_8:9	C902	RM11448-RM11549	23 lines for ZS97, 28 lines for MY46	May-September, 2012
BC₂F_8:9	C903	RM1232-RM11615	24 lines for ZS97, 27 lines for MY46	May-September, 2012
BC₂F_10:11	C1001	RM11543-RM11554	30 lines for ZS97, 30 lines for MY46	April-August, 2013
BC₂F_10:11	C1002	RM11569-RM11625	30 lines for ZS97, 30 lines for MY46	April-August, 2013

Generation	NIL name	Segregating region	Sample for two homozygous genotypes	Growing season
BC₂F_8:9	C901	RM11448-RM11522	24 lines for ZS97, 26 lines for MY46	May-September, 2012
BC₂F_8:9	C902	RM11448-RM11549	23 lines for ZS97, 28 lines for MY46	May-September, 2012
BC₂F_8:9	C903	RM1232-RM11615	24 lines for ZS97, 27 lines for MY46	May-September, 2012
BC₂F_10:11	C1001	RM11543-RM11554	30 lines for ZS97, 30 lines for MY46	April-August, 2013
BC₂F_10:11	C1002	RM11569-RM11625	30 lines for ZS97, 30 lines for MY46	April-August, 2013

Trait	NIL name	Values of the two homozygous genotypic groups (Mean ± SD)		P	A	R² (%)
Trait	NIL name	Zhenshan 97	Milyang 46	P	A	R² (%)
TGW (g)	C901	27.08 ± 0.26	27.10 ± 0.33	0.8877
	C902	27.49 ± 0.21	27.24 ± 0.34	0.0033	-0.12	10.55
	C903	27.60 ± 0.34	27.32 ± 0.35	0.0065	-0.14	8.3
	C1001	24.68 ± 0.37	24.43 ± 0.28	0.0043	-0.13	15.19
	C1002	24.59 ± 0.71	24.76 ± 0.61	0.3269
GL (mm)	C901	8.658 ± 0.036	8.640 ± 0.035	0.0653
	C902	8.888 ± 0.052	8.888 ± 0.063	0.9646
	C903	8.819 ± 0.038	8.754 ± 0.045	< 0.0001	-0.032	23.93
	C1001	7.951 ± 0.064	7.915 ± 0.057	0.0297	-0.018	8.57
	C1002	7.904 ± 0.114	7.929 ± 0.100	0.3882
GW (mm)	C901	3.002 ± 0.017	2.998 ± 0.017	0.5798
	C902	2.980 ± 0.017	2.960 ± 0.018	< 0.0001	-0.011	17.3
	C903	2.978 ± 0.019	2.961 ± 0.020	0.0018	-0.009	11.73
	C1001	2.913 ± 0.024	2.906 ± 0.019	0.2231
	C1002	2.921 ± 0.032	2.918 ± 0.029	0.8199
HD (d)	C901	62.7 ± 0.8	62.4 ± 0.8	0.2234
	C902	58.8 ± 0.6	59.0 ± 0.6	0.3662
	C903	63.0 ± 1.1	63.4 ± 0.8	0.127
	C1001	74.5 ± 0.6	74.5 ± 0.7	0.9086
	C1002	72.3 ± 0.9	72.1 ± 0.7	0.3852

Trait	NIL name	Values of the two homozygous genotypic groups (Mean ± SD)		P	A	R² (%)
Trait	NIL name	Zhenshan 97	Milyang 46	P	A	R² (%)
TGW (g)	C901	27.08 ± 0.26	27.10 ± 0.33	0.8877
	C902	27.49 ± 0.21	27.24 ± 0.34	0.0033	-0.12	10.55
	C903	27.60 ± 0.34	27.32 ± 0.35	0.0065	-0.14	8.3
	C1001	24.68 ± 0.37	24.43 ± 0.28	0.0043	-0.13	15.19
	C1002	24.59 ± 0.71	24.76 ± 0.61	0.3269
GL (mm)	C901	8.658 ± 0.036	8.640 ± 0.035	0.0653
	C902	8.888 ± 0.052	8.888 ± 0.063	0.9646
	C903	8.819 ± 0.038	8.754 ± 0.045	< 0.0001	-0.032	23.93
	C1001	7.951 ± 0.064	7.915 ± 0.057	0.0297	-0.018	8.57
	C1002	7.904 ± 0.114	7.929 ± 0.100	0.3882
GW (mm)	C901	3.002 ± 0.017	2.998 ± 0.017	0.5798
	C902	2.980 ± 0.017	2.960 ± 0.018	< 0.0001	-0.011	17.3
	C903	2.978 ± 0.019	2.961 ± 0.020	0.0018	-0.009	11.73
	C1001	2.913 ± 0.024	2.906 ± 0.019	0.2231
	C1002	2.921 ± 0.032	2.918 ± 0.029	0.8199
HD (d)	C901	62.7 ± 0.8	62.4 ± 0.8	0.2234
	C902	58.8 ± 0.6	59.0 ± 0.6	0.3662
	C903	63.0 ± 1.1	63.4 ± 0.8	0.127
	C1001	74.5 ± 0.6	74.5 ± 0.7	0.9086
	C1002	72.3 ± 0.9	72.1 ± 0.7	0.3852

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