Development of New Submergence Tolerant Rice Variety for Bangladesh Using Marker-Assisted Backcrossing

doi:10.1016/S1672-6308(14)60277-8

Abstract

Abstract:

Submergence tolerant high yielding rice variety was developed using BR11 as a recipient parent applying foreground, phenotypic and background selection approaches. Recombinant selection was found essential to minimize linkage drag by BC₂F₂ generation. Without recombinant selection, the introgression size in the backcross recombinant lines (BRLs) was approximately 15 Mb on the carrier chromosome. The BRLs were found submergence tolerance compared to the check varieties under complete submergence for two weeks at Bangladesh Rice Research Institute, and produced higher yield compared to the isogenic Sub1-line under controlled submerged condition. The BRL IR85260-66-654-Gaz2 was released as BRRI dhan52 in 2010, which was the first high yielding submergence tolerant variety in Bangladesh. BRRI dhan52 produced grain yield ranging from 4.2 to 5.2 t/hm² under different flash flood prone areas of Bangladesh in three consecutive seasons. The study demonstrated the efficiency of recombinant selection and better adaptability of the newly released submergence tolerant high yielding variety in flash flood prone different areas of the country with respect to submergence tolerance and yield potential.

Key words: backcross recombinant line, marker-assisted backcrossing, recombinant selection, rice, submergence tolerance

Md Iftekharuddaula Khandakar, Uddin Ahmed Helal, Ghosal Sharmistha, Rahman Moni Zakiah, Amin Al, Shamsher Ali Md. Development of New Submergence Tolerant Rice Variety for Bangladesh Using Marker-Assisted Backcrossing[J]. Rice Science, 2015, 22(1): 16-26.

Figures/Tables 11

References 22

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Plant No.	A	H	B	Recipient allele (%)	Rank-background
14	46	26	0	81.9	2
59	26	46	0	68.1	7
73	23	41	8	60.4	9
86	29	43	0	70.1	5
145	34	38	0	73.6	4
235	18	43	11	54.9	10
248	26	46	0	68.1	8
396	48	24	0	83.3	1
445	44	28	0	80.6	3
496	27	45	0	68.8	6
Average		35		71

Plant No.	A	H	B	Recipient allele (%)	Rank-background
14	46	26	0	81.9	2
59	26	46	0	68.1	7
73	23	41	8	60.4	9
86	29	43	0	70.1	5
145	34	38	0	73.6	4
235	18	43	11	54.9	10
248	26	46	0	68.1	8
396	48	24	0	83.3	1
445	44	28	0	80.6	3
496	27	45	0	68.8	6
Average		35		71

Plant No.	A	H	B	Recipient allele (%)	Rank-background
396-2	59	13	0	89.8	4
396-18	60	12	0	90.6	3
396-26	64	8	0	93.8	2
396-37	59	13	0	89.8	5
396-45	55	17	0	86.7	7
396-66	67	5	0	96.1	1
396-89	58	14	0	89.1	6
Average		12		90.8

Plant No.	A	H	B	Recipient allele (%)	Rank-background
396-2	59	13	0	89.8	4
396-18	60	12	0	90.6	3
396-26	64	8	0	93.8	2
396-37	59	13	0	89.8	5
396-45	55	17	0	86.7	7
396-66	67	5	0	96.1	1
396-89	58	14	0	89.1	6
Average		12		90.8

Marker	Plant number
Marker	396-158	396-159	396-217	396-258	396-294	396-559	396-607	396-642	396-654	396-664	396-769	396-771
RM237	A	A	A	A	A	A	A	A	A	A	A	A
RM486	A	A	A	A	A	A	A	A	A	A	A	A
RM154	A	A	A	A	A	A	A	A	A	A	A	A
RM279	A	A	A	A	A	A	A	A	A	A	A	A
RM566	B	B	B	B	B	B	B	H	B	B	H	B