Rice Science

Rice Science

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Histone Acetyltransferase GCN5 Regulates Rice Growth and Development and Enhances Salt Tolerance

  1. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding / Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops / Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China; School of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Zhenjiang 212499, China; #These authors contributed equally to this work
  • Contact: GONG Zhiyun
  • Supported by:

    This work was supported by the Key R&D Program of Jiangsu Province (Grant No. BE2022335), the Jiangsu Province Government (Grant No. JBGS[2021]001), the Natural Science Foundation of Jiangsu Province (Grant No. BK20230295), the Project of Zhongshan Biological Breeding Laboratory (Grant No. BM2022008-02), Outstanding Youth Fund of Jiangsu Province (Grant No. BK20230013), and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Abstract: Histone acetylation, is indispensable in the process of crops resisting abiotic stress, which is jointly catalyzed by histone acetyltransferase and deacetylase. However, the mechanism of regulating salt tolerance through histone acetyltransferase GCN5 is still unclear. We reveal that OsGCN5 can catalyze the acetylation of canonical H3 and H4 lysine sites in vivo and in vitro in rice. The knockout mutants and RNAi lines of OsGCN5 exhibited severe growth inhibition and defects in salt tolerance, while the overexpression of OsGCN5 enhanced the salt tolerance of rice seedlings, indicating that OsGCN5 positively regulates the response process of rice to salt stress. The RNA-seq analysis suggest OsGCN5 may positively regulate the salt tolerance of rice by inhibiting the expression of OsHKT2;1, or other salt-responsive genes. Taken together, our study indicates that GCN5 plays a key role in the preservation of salt tolerance in rice.

Key words: GCN5; histone acetyltransferase, salt tolerance, Oryza sativa