Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis

doi:10.1016/S1672-6308(08)60057-8

摘要/Abstract

摘要： As maternal parents, diploid (L202-2x) and autotetraploid (L202-4x) of Oryza sativa cv. L202 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were double- fertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of double- and single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

关键词: Oryza sativa, Oryza officinalis, diploid rice, autotetraploid rice, wild rice, interspecific hybridization, embryo development, infertility

FU Xue-lin, LU Yong-gen, LIU Xiang-dong, LI Jin-quan, ZHAO Xing-juan. Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis[J]. RICE SCIENCE, 2009, 16(1): 58-64 .

参考文献

1 Bellon M R, Brar D S, Lu B R, Pham J L. Rice genetic resources. In: Dowling N G, Greenfield S M, Fischer K S. Sustainability of Rice in the Global Food System. Manila: International Rice Research Institute, 1999: 251–283.
2 He G C. Exploration and transfer of elite genes of wild rice. In: Luo L J, Ying C S, Tang S X. Rice Germplasm. Wuhan: Hubei Science and Technology Publisher, 2002: 279. (in Chinese)
3 Barclay A. Cultivated rice receives help from wild cousins. Appropriate Technol, 2004, 31(4): 10–12.
4 Qin X Y, Zhu R C, Tang J H, Li W K, Li D Y, Wei S M, Huang F K. Genetic analysis and utilization of brown planthopper (BPH)-resistance genes in Oryza officinalis Wall. J Plant Genet Res, 2007, 8(1): 41–45. (in Chinese with English abstract)
5 Jena K K, Khush G S. Introgression of genes from Oryza officinalis Well ex Watt to cultivated rice, O. sativa L. Theor Appl Genet, 1990, 80: 737–745.
6 Yan H H, Hu H Y, Fu Q, Yu H Y, Tang S X, Xiong Z M, Min S K. Morphological and cytogenetical studies of the hybrids between Oryza sativa and Oryza officinalis. Chinese J Rice Sci, 1996, 10(3): 138–142. (in Chinese with English abstract)
7 Zhong D B, Luo L J, Guo L B, Ying C S. Studies on resistance to brown planthopper (BPH) of hybrid between Oryza sativa and Oryza officinalis. Southwest China J Agric Sci, 1997, 10(2): 5–9. (in Chinese with English abstract)
8 Huang Z, He G C, Shu L, Li X, Zhang Q. Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet, 2001, 102: 929–934.
9 Tan G X, Weng Q M, Ren X, Huang Z, Zhu L L, He G C. Two whitebacked planthopper resistance genes in rice share the same loci with those for brown planthopper resistance. Heredity, 2004, 92: 212–217.
10 Tan G X, Ren X, Weng Q M, Shi Z Y, Zhu L L, He G C. Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genet Sin, 2004, 31(7): 724–729. (in Chinese with English abstract)
11 Tan G X, Jin H J, Li G, He R, Zhu L, He G. Production and characterization of a complete set of individual chromosome additions from Oryza officinalis to Oryza sativa using RFLP and GISH analyses. Theor Appl Genet, 2005, 111: 1585–1595.
12 Tang M, Jin H J, Tan G X, Zhu L L, He G C. Analysis of a mosaic monosomic alien addition line of rice with chromosome 7 of Oryza officinalis C-genome and its backcross progeny. J Wuhan Bot Res, 2005, 23(3): 221–226. (in Chinese with English abstract)
13 Yu W J, Luo K, Guo X X. Obtaining the hybrids of Oryza sativa×O. officinalis without embryo culture by using male-sterile lines. Acta Genet Sin, 1993, 20(4): 348–353. (in Chinese with English abstract)
14 Hu S Y. Method of preparation of slides used to examine the pollen germination on the stigma and pollen tube growth in the style. Chin Bull Bot, 1994, 11: 58–60. (in Chinese with English abstract)
15 Itoh J I, Nonomura K I, Ikeda K, Yamaki S, Inukai Y, Yamagishi H, Kitano H, Nagato Y. Rice plant development: from zygote to spikelet. Plant Cell Physiol, 2005, 46(1): 23–47.
16 Cai D T, Yuan L, Lu X G. A new strategy of rice breeding in the 21st century: II. Searching a new pathway of rice breeding by utilization of double heterosis of wide cross and poly- ploidization. Acta Agron Sin, 2001, 27(1): 110–116. (in Chinese with English abstract)
17 Fu X L, Lu Y G, Liu X D, Li J Q, Feng J H. Cytological mechanisms of interspecific incrossability and hybrid sterility between Oryza sativa L. and O. alta Swallen. Chin Sci Bull, 2007, 52 (6): 755–765.
18 Fu X L, Lu Y G, Liu X D, Li J Q. Progress on transferring elite Genes from non-AA genome wild rice into Oryza sativa through interspecific hybridization. Rice Sci, 2008, 15(2): 79–87.
19 Multani D S, Jena K K, Brar D S, de los Reyes B G, Angeles E R, Khush G S. Development of monosomic alien addition lines and introgression of genes from Oryza australiensis Domin, to cultivated rice of O. sativa L. Theor Appl Genet, 1994, 88: 102–109.
20 Yasui H, Iwata N. Production of monosomic alien addition lines of Oryza sativa having a single O. punctata chromosome. In: Khush G S. Rice Genetics II. Manila: International Rice Research Institute, 1991: 147–155.

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