Rice Science ›› 2022, Vol. 29 ›› Issue (6): 569-576.DOI: 10.1016/j.rsci.2022.01.011
收稿日期:
2021-10-22
接受日期:
2022-01-26
出版日期:
2022-11-28
发布日期:
2022-09-09
. [J]. Rice Science, 2022, 29(6): 569-576.
Site | Trait | 9311 (Mean ± SD, n = 5) | Backcross inbred line | ||
---|---|---|---|---|---|
Mean ± SD (n = 5) | CV (%) | Range | |||
Ezhou | Pn [µmol/(m2·s)] | 19.90 ± 1.00 | 22.00 ± 5.70 | 25.90 | 10.30-36.20 |
Gs [mol/(m2·s)] | 0.75 ± 0.06 | 0.68 ± 0.19 | 27.91 | 0.30-1.21 | |
BM (g) | 41.17 ± 1.11 | 55.89 ± 9.43 | 16.72 | 23.95-89.00 | |
DBM (g/d) | 0.29 ± 0.01 | 0.39 ± 0.06 | 16.70 | 0.06-0.61 | |
Lingshui | Pn [µmol/(m2·s)] | 25.00 ± 0.69 | 26.20 ± 3.40 | 12.83 | 17.20-32.60 |
Gs [mol/(m2·s)] | 0.85 ± 0.13 | 0.80 ± 0.22 | 27.82 | 0.31-1.32 | |
BM (g) | 33.31 ± 4.75 | 56.25 ± 8.21 | 15.01 | 34.05-73.98 | |
DBM (g/d) | 0.24 ± 0.03 | 0.41 ± 0.06 | 15.14 | 0.06-0.54 |
Table 1. Pn and BM of backcross inbred lines in different sites.
Site | Trait | 9311 (Mean ± SD, n = 5) | Backcross inbred line | ||
---|---|---|---|---|---|
Mean ± SD (n = 5) | CV (%) | Range | |||
Ezhou | Pn [µmol/(m2·s)] | 19.90 ± 1.00 | 22.00 ± 5.70 | 25.90 | 10.30-36.20 |
Gs [mol/(m2·s)] | 0.75 ± 0.06 | 0.68 ± 0.19 | 27.91 | 0.30-1.21 | |
BM (g) | 41.17 ± 1.11 | 55.89 ± 9.43 | 16.72 | 23.95-89.00 | |
DBM (g/d) | 0.29 ± 0.01 | 0.39 ± 0.06 | 16.70 | 0.06-0.61 | |
Lingshui | Pn [µmol/(m2·s)] | 25.00 ± 0.69 | 26.20 ± 3.40 | 12.83 | 17.20-32.60 |
Gs [mol/(m2·s)] | 0.85 ± 0.13 | 0.80 ± 0.22 | 27.82 | 0.31-1.32 | |
BM (g) | 33.31 ± 4.75 | 56.25 ± 8.21 | 15.01 | 34.05-73.98 | |
DBM (g/d) | 0.24 ± 0.03 | 0.41 ± 0.06 | 15.14 | 0.06-0.54 |
Fig. 1. Frequency distributions of photosynthetic rate (A), stomatal conductance (B), biomass (C) and daily biomass (D) of backcross inbred lines (BILs) in different sites.
Trait | Pn | Gs | BM | DBM |
---|---|---|---|---|
Pn | 0.437** | 0.168* | 0.174* | |
Gs | 0.588** | -0.042 | -0.022 | |
BM | 0.202* | -0.049 | 0.988** | |
DBM | 0.256** | -0.011 | 0.958** |
Table 2. Correlation coefficients among photosynthesis and biomass of backcross inbred lines in Ezhou (upper right) and Lingshui (lower left), China.
Trait | Pn | Gs | BM | DBM |
---|---|---|---|---|
Pn | 0.437** | 0.168* | 0.174* | |
Gs | 0.588** | -0.042 | -0.022 | |
BM | 0.202* | -0.049 | 0.988** | |
DBM | 0.256** | -0.011 | 0.958** |
Fig. 2. QTL analysis of photosynthesis of backcross inbred line population in different sites. Upward direction indicates the allele showing positive effects, while downward direction indicates the allele showing negative effects. Pn, Photosynthetic rate; Gs, Stomatal conductance; BM, Biomass; DBM, Daily biomass.
QTL | Site | Chr. | L/Bin | R/Bin | LOD | PVE (%) | Add |
---|---|---|---|---|---|---|---|
qPn1.1 | Lingshui | 1 | 1-143 | 1-144 | 3.5 | 11.9 | -1.1381 |
qPn2.1 | Lingshui | 2 | 2-25 | 2-26 | 2.9 | 9.2 | -1.1851 |
qPn5.1 | Ezhou | 5 | 5-86 | 5-87 | 3.1 | 7.9 | -2.6304 |
qPn8.1 | Ezhou | 8 | 8-85 | 8-86 | 3.6 | 9.2 | 3.2571 |
Lingshui | 8 | 8-85 | 8-86 | 2.7 | 8.4 | 1.5921 | |
qPn12.1 | Ezhou | 12 | 12-121 | 12-122 | 2.8 | 7.0 | -1.7882 |
qGs2.1 | Ezhou | 2 | 2-256 | 2-257 | 3.9 | 11.7 | -0.1334 |
qBM1.1 | Ezhou | 1 | 1-143 | 1-144 | 3.0 | 8.2 | 3.1844 |
Lingshui | 1 | 1-143 | 1-144 | 4.5 | 15.0 | 3.6097 | |
qBM6.1 | Ezhou | 6 | 6-107 | 6-108 | 3.0 | 8.1 | 5.2379 |
qDBM1.1 | Ezhou | 1 | 1-143 | 1-144 | 4.5 | 8.1 | 0.0243 |
Lingshui | 1 | 1-143 | 1-144 | 3.4 | 13.0 | 0.0240 | |
qDBM3.1 | Ezhou | 3 | 3-179 | 3-180 | 2.7 | 4.5 | -0.0326 |
qDBM4.1 | Ezhou | 4 | 4-99 | 4-100 | 4.4 | 7.7 | -0.0332 |
qDBM6.1 | Ezhou | 6 | 6-106 | 6-107 | 5.3 | 9.3 | 0.0422 |
qDBM12.1 | Ezhou | 12 | 12-54 | 12-55 | 3.0 | 5.0 | -0.0173 |
Table 3. QTL information for photosynthic rate and biomass of backcross inbred lines in fields.
QTL | Site | Chr. | L/Bin | R/Bin | LOD | PVE (%) | Add |
---|---|---|---|---|---|---|---|
qPn1.1 | Lingshui | 1 | 1-143 | 1-144 | 3.5 | 11.9 | -1.1381 |
qPn2.1 | Lingshui | 2 | 2-25 | 2-26 | 2.9 | 9.2 | -1.1851 |
qPn5.1 | Ezhou | 5 | 5-86 | 5-87 | 3.1 | 7.9 | -2.6304 |
qPn8.1 | Ezhou | 8 | 8-85 | 8-86 | 3.6 | 9.2 | 3.2571 |
Lingshui | 8 | 8-85 | 8-86 | 2.7 | 8.4 | 1.5921 | |
qPn12.1 | Ezhou | 12 | 12-121 | 12-122 | 2.8 | 7.0 | -1.7882 |
qGs2.1 | Ezhou | 2 | 2-256 | 2-257 | 3.9 | 11.7 | -0.1334 |
qBM1.1 | Ezhou | 1 | 1-143 | 1-144 | 3.0 | 8.2 | 3.1844 |
Lingshui | 1 | 1-143 | 1-144 | 4.5 | 15.0 | 3.6097 | |
qBM6.1 | Ezhou | 6 | 6-107 | 6-108 | 3.0 | 8.1 | 5.2379 |
qDBM1.1 | Ezhou | 1 | 1-143 | 1-144 | 4.5 | 8.1 | 0.0243 |
Lingshui | 1 | 1-143 | 1-144 | 3.4 | 13.0 | 0.0240 | |
qDBM3.1 | Ezhou | 3 | 3-179 | 3-180 | 2.7 | 4.5 | -0.0326 |
qDBM4.1 | Ezhou | 4 | 4-99 | 4-100 | 4.4 | 7.7 | -0.0332 |
qDBM6.1 | Ezhou | 6 | 6-106 | 6-107 | 5.3 | 9.3 | 0.0422 |
qDBM12.1 | Ezhou | 12 | 12-54 | 12-55 | 3.0 | 5.0 | -0.0173 |
Fig. 3. Validation of function of qPn1.1/qBM1.1/qDBM1.1. A, Comparison of genotypic structure of qPn1.1/qBM1.1/qDBM1.1 locus in different backcross inbred lines (BILs). Black bar and blank bar represent fragments from O. longistaminata and 9311, respectively. B, Photosynthetic rate of corresponding BILs. Different lowercase letters at the top of the bar indicate significant differences among different BILs by the Duncan’s multiple range test at P < 0.05.
Fig. 4. Validation of function of qPn8.1. A, Comparison of genotypic structure of qPn8.1 locus in different backcross inbred lines (BILs). Black bar and blank bar represent fragments from O. longistaminata and 9311, respectively. Gray bar represents heterozygote genotype. B, Photosynthetic rate of corresponding BILs. Different lowercase letters at the top of the bar indicate significant differences among different BILs at P < 0.05 by the Duncan’s multiple range test. C, Means of Pn of BILs with qPn8.1. ***, Significant difference at P < 0.001 by the Duncan’s multiple range test. D, Relative expression levels of candidate gene ARE1 in different BILs and 9311. In A, B and D, BIL1763, BIL1781, BIL1728, BIL1731 and BIL1787 harbor qPn8.1 from O. longistaminata; BIL1702 does not harbor qPn8.1 as 9311; and BIL1711 is the heterozygote genotype.
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